Results
NOBERRYVIRUS - Results
The project led to the development of two certified real-time detection kits for known and newly discovered raspberry viruses, the long-term preservation of a five virus isolates (three raspberry viruses, two strawberry viruses), the storage of various varieties of healthy strawberries and raspberries in cryobanks and tissue cultures in the Czech Republic and Norway, and to the preparation of five methodologies and one utility model for the detection of selected viruses, cryopreservation of their isolates and cryopreservation therapy of plants.
The scientific results of the project were described in 7 scientific publications (additional 3 under-review and 2 being newly prepared) and another 34 contributions in proceedings, journals/newspapers, or scientific conferences.
The results are detailed in the list below.
Functional samples and isolates:
- 2021: The isolate of RLBV (NPGZM – https://www.microbes.cz/collections/vurv.exe/search, functional sample no. UPOC-VIR-049)
- 2022: Detection kit (RubusVir I qPCR-RG Detection Kit) – a kit for a real-time PCR detection of BRNV, RBDV, RpRSV, and SLRSV viruses in plant samples (available upon request at RBIP Holovousy)
- 2023: Detection kit (RubusVir II qPCR-RG Detection Kit – a kit for a real-time PCR detection of RaEV1, RaRV1, and RYNV in plant samples (available upon request at RBIP Holovousy)
- 2024: Cryopreserved viruses in their host plants - raspberry with RVCV, RBDV, RaRV1 viruses (CRI Cryobank, Czech Republic), strawberry with SVBV a SMYEV viruses (NIBIO cryobank, Norway)
- 2024: Functional sample: Virus-tested strawberry and raspberry nuclear stocks is stored in the Jan Holub, Ltd. facilities, CRI Cryobank (Czech Republic) and Sagaplant (Norway)
Methodologies:
- 2022: Valentová L., Rejlová M., Čmejla R., 2022. Real-time PCR detection of black raspberry necrosis virus (BRNV), raspberry ringspot virus (RpRSV), raspberry bushy dwarf virus (RBDV) a strawberry latent ringspot virus (SLRSV) in biological material
- 2023: Bilavčík A., Bobrova O., Faltus M., Fránová J., Hammond S., Koloniuk I., Zámečník J. 2023a. Metodika terapie virových patogenů pomocí kryoprezervace (Virus eradication method by cryopreservation tool - a cryoknife). Crop Research Institute, Prague, Czech Republic, ISBN:987-80-7427-418-3
- 2023: Bilavcik A., Bjerkestrand R., Bjelland B., Blystad D.-R., Bobrova O., Franova J., Faltus M., Hamborg Z., Hammond S., Koloniuk I., Zamecnik J. 2023b. Methodology for co-cryostorage of virus isolates in their host plants and relevant data. Crop Research Institute, Prague, Czech Republic, ISBN:987-80-7427-417-6
- 2024: Valentová L., Rejlová M., Čmejla R., 2024. Real-time PCR detekce virů Rubus yellow net virus (RYNV), raspberry rubodvirus 1 (RaRV1) a raspberry enamovirus 1 (RaEV1) v rostlinném materiálu. VŠÚO, Holovousy, Česká republika, ISBN 978-80-87030-98-
- 2024: Bilavčík A., Bobrova O., Faltus M. Zámečník J. 2024 Metodika kryoprezervace dormantních pupenů maliníku (Methodology for dormant berry bud cryopreservation) (CRI) ISBN: 978-80-7427-429-9
Utility models:
- 2023: Utility model no. 37208: Sada pro detekci virů raspberry virus 1 a raspberry virus 2 v biologickém materiálu
Scientific publications:
- 2022: Tan J.L., Trandem N., Fránová J., Hamborg Z., Blystad D.-R., Zemek R. 2022. Known and potential invertebrate vectors of raspberry viruses. Viruses 14(3):571.
- 2023: Koloniuk, I., Fránová J., Přibylová J., Sarkisova T., Špak J., Tan J.L., Zemek R., Čmejla R., Rejlová M., Valentová L., Sedlák J., Holub J., Skalík J., Blystad D.-R., Sapkota B., Hamborg Z. (2023) Molecular characterization of a novel enamovirus infecting raspberry. Viruses 15(2), 2281.
- 2024: Bilavčík A., Hammond S.D.H., Franova J., Koloniuk I., Hamborg Z., Blystad D., Faltus M., Zamecnik J., 2024 Elimination of black raspberry necrosis virus from raspberry (Rubus idaeus L.) by in vitro cryotherapy, European Journal of Horticultural Science (v tisku).
- 2024: Tan J.L., Trandem N., Hamborg Z., Fránová J., Blystad D.-R., Zemek R. 2024. Thrips species occurring in red raspberry, Rubus idaeus L., in South Norway. Spanish Journal of Agricultural Research, 22 (3), e10SC01.
- 2024: Tan J.L., Trandem N., Hamborg Z., Sapkota B., Blystad D.-R., Fránová J., Zemek R. 2024. The species, density, and intra-plant distribution of mites on red raspberry (Rubus idaeus L.), Experimental and Applied Acarology, 93 (2): 317-337.
- 2024: Valentová L., Rejlová M., Čmejla R. 2024. New methods for the detection of viruses infecting Rubus plants - verification in diagnostic practice. Vědecké práce ovocnářské 30(1): 1-11
- 2024: Lenz O., Koloniuk I., Sarkisová T., Čmejla R., Valentová L., Rejlová M., Sedlák J., Blystad D-R., Sapkota B., Hamborg Z., Tan J., Zemek R., Přibylová J., Fránová J. (2024) Molecular characterization of a novel rubodvirus infecting raspberries. Viruses 16(7): 1074.
Other results:
- see separate file [PDF]
