Research

Research of the Department of Plant Virology focuses on molecular pathogenes of plants - viruses, phytoplasmas, and bacteria.

Viruses

  • Development of new molecular methods for viruses detection (real-time PCR, microarrays)
  • Screening for novel viruses
  • Virus transmission by mechanical inoculation, by aphids and grafting
  • Immunisation and antisera preparation with natural or recombinant antigens
  • Detection of viruses by nucleic acid based and ELISA methods
  • Analysis and expressions of virus genomes
  • Study of molecular variability and evolution of comoviruses
  • Finding, identification and sequencing of the genome of bacteriophages and their use in plant protection
  • Expression of phage lytic enzymes as a means against antibiotic-resistant bacteria
  • dr. I.Koloniuk and prof. K.Petrzik are members of Hypoviridae Study Group, and Secoviridae Study Group, respectively, of the International Committee on Taxonomy of Viruses (ICTV)
  • prof. K.Petrzik is a section editor of Virus Genes journal (Springer)

 

Phytoplasmas

  • Detection, identification and characterization of phytoplasmas based on PCR amplification, RFLP analysis, multilocus gene sequencing, dodder transmission to periwinkle, and transmission electron microscopy
  • Development of new molecular technique for phytoplasmas detection (microarrays)
  • Study of phytopathogenes occurrence and its genetic variants
  • Screening and genome analysis of phytoplasma plasmids

CONTACT

Biology Centre CAS
Institute of Plant Molecular Biology
Branišovská 1160/31
370 05 České Budějovice

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